|
KMID : 0364819880260030149
|
|
Korean Journal of Microbiology
1988 Volume.26 No. 3 p.149 ~ p.154
|
|
|
Detection of Japanese Encephalitis Virus by Biotinylated cDNA Probe
|
|
Hwang, Dong Youn/황동연
Shin, Yung Oh/Yim, Jeong Bin/신영오/임정빈
|
|
|
Abstract
|
|
|
1
Japanese Encephalitis Virus (JEV) can be detected conveniently by the an of blotinylated cDNA probe. To prepare blotinylated probe aminoallyl dUTP was first synthesised chemically to reverse transcribe the viral RNA. The allylamiae-labeled cDNA was them converted to the biotin-cDNA by the reaction with an activated biotin ester, NHS-ACA-biotin. The JEV geomic RNA was hybridized to the biotiaylated cDNA probe on nitrocellulose filter and visualized colorimetrkaly by streptavidin complexes with alkaline phosphatase polymer. Sensitivity of the detection system was determined by estimating the amount of the JEV genomic RNA through comparison with signals generated from the biodnylatcd and 32P-labeled probes. It was found that the biota probe was as sensitive as 32P-cDNA probe which can detect 50 pgs of the target RNA.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|